CO-CULTURE OF DISSOCIATED HIPPOCAMPAL AND SYMPATHETIC CELLS FROM THE NEONATAL RAT

The Culture of Dissociated Cells from Rat Cerebral Cortex

Co-culture of rat neonatal ventricular myocytes and GFP-labeled feline c-Kit cells: Neonatal rat ventricular myocytes

BrdU infusion into normal cats: Osmotic pumps (ALZET) were filled with 50mg/mL BrdU dissolved in 50% DMSO/50% ddH2O filtered through a sterile 0.22μm filter. Pumps were then incubated in sterile saline at 37 °C overnight prior to insertion. The pumps were surgically inserted subcutaneously just caudal to the scapulae per the protocol approved by IACUC at Temple University. The number of pumps w...

Culture and Expansion of Rat Mesenchymal Stem Cells Using the Serum Prepared From Rat’S Peripheral Blood

Purpose: The aim of this study is to evaluate the effects of prepared serum from rat peripheral blood on growth and viability of rat bone marrow-derived mesenchymal stem cells (MSCs) in vitro. Materials and Methods: Bone marrow cells prepared from rat’s tibia and femur central canal were cultured either in medium containing fetal bovin serum (FBS) or rat peripheral blood-derived serum (PBDS) f...

Evidence for serotonin synthesis, uptake, and release in dissociated rat sympathetic neurons in culture.

In the previous paper (Matsumoto et al., 1987), pharmacological evidence for secretion of serotonin (5-HT) by cultured sympathetic principal neurons was reported. Here, we present further evidence that 5-HT is a transmitter of some of these neurons under certain culture conditions, and can also be a "false" transmitter. Sympathetic principal neurons, dissociated from superior cervical ganglia (...

the role of russia in transmission of energy from central asia and caucuses to european union

پس ازفروپاشی شوروی،رشد منابع نفت و گاز، آسیای میانه و قفقاز را در یک بازی ژئوپلتیکی انرژی قرار داده است. با در نظر گرفتن این منابع هیدروکربنی، این منطقه به یک میدانجنگ و رقابت تجاری برای بازی های ژئوپلتیکی قدرت های بزرگ جهانی تبدیل شده است. روسیه منطقه را به عنوان حیات خلوت خود تلقی نموده و علاقمند به حفظ حضورش می باشد تا همانند گذشته گاز طبیعی را به وسیله خط لوله مرکزی دریافت و به عنوان یک واس...